The ultimate source of many of these genes is almost certainly the actinomycetes that make the antibiotics and therefore need self‐protective mechanisms to avoid suicide. How do they ensure that they are resistant at the time when intracellular antibiotic concentrations reach potentially lethal levels? Antibiotics, as typical secondary metabolites, are usually not produced during […]
Antibiotic Producing Bacteria; Self-resistance Read More »
Organic solvents were used to either precipitate enzyme or to study denaturation. The reason why nonaqueous enzymology has generated so much excitement is that enzymes exhibit striking new properties in organic solvents. For example, in organic solvents enzymes often catalyze reactions impossible in water and are very stable. Furthermore, enzyme selectivity in organic solvents is
Enzyme Function in Organic Solvent Read More »
The use of fluorescence in situ hybridization (FISH) has revealed that each chromosome occupies a distinct nuclear territory and that the 3D positioning of chromosomes correlates with chromosome size and gene density. “Think about it, where larger chromosomes should reside, where gene poor chromosomes can be, what kind of chromosomes should occupy centre of
Functional Organization of Genome Read More »
Argonaute protein is involved in RNAi, it recognizes and binds to siRNA duplex, cleaves and removes passenger strand, and allows guide strand to scan target mRNA. Argonaute (AGO) proteins have 4 discrete folding units: N-terminal: Acts as a wedge. PAZ domain: contains a binding pocket, anchors the 2-nucleotide 3′ overhang of siRNA like duplex. MID
Guide and passenger strand recognition by Argonaute protein Read More »